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Innovative Glycoproteomic Solution Accessible to Any Lab
The study of glycoproteomics presents researchers with costly analytical challenges due to the complexity and size of individual glycoomes. When you consider the number of biologic interactions, the complexity grows exponentially from nucleome to proteome to glycome. Researchers’ need for information has outpaced currently available technology. For example, image to the right shows diversity of glycoforms of a single human alpha-acid glycoprotein. These forms correlate with health status of an individual.
Our patent pending NAnoplasmonic Proximity Assay (NAPA) Technology Platform developed by Glycodots LLC addresses this complexity and the need for more information by combining label-free, real-time detection methods with high density multi-well plate assay formats. NAPA Technology Platform utilizes nanoplasmonic phenomenon, which provides a cost effective, high throughput solution for detecting and quantifying the binding of potentially thousands of glycoproteins in a single experiment.
This cost efficient assay for studying carbohydrate protein interactions in plate based format may serve as viable alternative for both lectin arrays and conventional SPR technologies. Most important it requires nothing but a Kit provided by Glycodots LLC and a regular absorbance plate reader most likely available at any biochemistry lab for running an ELISA.
Kit Components
The GlycoSight® Kit contains a multiwell plate with lectins that recognize several different glycan structures: acetygalactosamiyl, mannosyl, galactosyl, fucosyl, sialic acid, acetyglucosamine, and neu5Ac. The kit also contains nanoparticles with detailed instructions how to conjugate your glycoprotein quickly and efficiently. The Kit also contains two reference analytes for the purpose of demonstrating a multitude of lectin binding abilities and practical applications. The appropriate buffers are supplied as well.
Applications
• Glycoform Characterization
• Binding kinetics determination
• Glycan structure determination from a single purified glycoprotein
• Glycoprotein identification with mass spectrum assistance
• Cancer related biomarkers
• Altered Glycan Structure
• New Glycoprotein Discovery
Features
• High-throughput, real-time, label-free LSPR-based assay
• Quantitative kinetic analysis
• Time and cost efficient
• Minimal sample quantity
• Minimal sample preparation
• Wild range and high-throughput applications for screening and biomarker discoveries
• No need for specialized equipment
